PHASE 17 · SMART MULTI-SITE ASSEMBLY

Design complex assemblies without an enterprise license.

Plan independent insertion sites, combinatorial construct libraries, Gibson or Golden Gate workflows, long-sequence partitioning and construct buildability in one research workspace.

Research use only. This tool provides planning heuristics for user-supplied DNA sequences. It does not guarantee cloning success and does not replace experimental validation.

Backbone

Paste the circular or linear backbone sequence and choose the preferred strategy.

Backbone name Strategy

Backbone DNA sequence

Overlap length (nt) Max synthesis fragment (nt) Circular backbone

Independent insertion sites

Add one or more positions. Each site can contain multiple alternative parts for combinatorial design.

Assembly plan

Strategy selection, part QC, internal Type IIS screening, partitioning and construct previews.

Add a backbone and at least one insertion site.

Handoff & export

Move a selected construct into the Plasmid Designer or export a reproducible planning record.