Phase 19 · Research-use only

Smart CRISPR Guide & Edit Validation Designer

Scan a user-supplied DNA sequence for SpCas9 or Cas12a guide candidates, rank them with transparent sequence-level checks, prioritize guides near a research target and continue to validation-primer design.

Guide selection · transparent ranking · validation workflow

Guide-design settings

Use an exact DNA sequence. A target position is optional but helps prioritize guides near a variant or intended edit region.

Design name Nuclease Target position (1-based, optional) Maximum candidates

DNA sequence 0 bp

This phase does not perform genome-wide off-target prediction. Exact guide reuse is screened only inside the sequence you provide.

Ready.

Guide-design overview

User Guide

Ranked guide candidates

Select one guide for downstream validation-primer planning. Ranking supports candidate prioritization and does not establish editing efficiency.

Rank	Guide	Strand	PAM	Score	GC	Cut anchor	Target distance	Sequence reuse	Warnings	Action

Selected guide & validation-planning workflow

How to interpret this phase

A high score means a candidate passed the current sequence-level heuristics reasonably well. It does not establish editing efficiency or genome-wide specificity. Confirm the intended reference sequence, PAM, guide orientation, genomic context and appropriate independent specificity evidence before laboratory use.