Phase 68 · qPCR analysis
ΔΔCt qPCR Analysis Calculator
Calculate ΔCt, ΔΔCt and relative expression (2−ΔΔCt) from target and reference-gene Ct values. Replicates are summarized by group with SD and SEM.
Input
Paste tab-, comma-, or semicolon-separated rows with four columns: Sample, Group, Target Ct, Reference Ct. Include one row per measurement.
Method
ΔCt = Cttarget − Ctreference · ΔΔCt = ΔCtsample − mean ΔCtcalibrator · Fold change = 2−ΔΔCt
Group summary
| Group | n | Mean ΔCt | SD ΔCt | SEM ΔCt | Mean ΔΔCt | 2^-ΔΔCt |
|---|---|---|---|---|---|---|
| No analysis yet. | ||||||
Per-measurement results
| Sample | Group | Target Ct | Reference Ct | ΔCt | ΔΔCt | Fold change |
|---|---|---|---|---|---|---|
| No analysis yet. | ||||||
Scientific limitations
- The comparative Ct method assumes comparable amplification efficiencies for target and reference assays.