Sequence Utilities
Clean DNA, generate reverse complements and RNA, translate all six reading frames, and identify basic start-to-stop ORFs.
Clean DNA
Reverse complement
RNA transcript (5′→3′)
Six-frame translation
Basic ORF finder
Reports ATG-to-stop ORFs on both strands. Coordinates are 1-based on the input DNA.
Sequence comparison & translation
Continue with pairwise alignment, sequence differences, six-frame translation and ORF analysis.
Sequence composition & quality
Inspect GC/AT composition, CpG positions, homopolymers and sliding-window complexity.
Open Sequence Composition & Quality AnalyzerCodon usage & optimization
Analyze coding-sequence codon use and optionally create a transparent synonymous reference redesign.
Open Codon Usage & Optimization AnalyzerMultiple Sequence Alignment & Conservation
Align three or more DNA, RNA or protein sequences with Clustal Omega and inspect conservation and pairwise identity.
Open Multiple Sequence Alignment