Phase 63 · DNA & RNA Tools
Parent Plasmid → Minicircle Mapper
Extract an intended retained circular region from a parent-plasmid sequence using explicit 1-based inclusive coordinates, including intervals that cross the displayed origin.
Parent plasmid sequence or FASTA
Optional final minicircle sequence for exact integrity comparison
Load demo Map retained minicircle
Ready.
Coordinate model Coordinates are 1-based and inclusive. If start ≤ end, the interval is direct. If start > end, the retained interval crosses the displayed parent origin. An optional final sequence can be compared exactly to the extracted retained sequence. This tool does not infer recombination sites or manufacturing chemistry. The retained sequence is defined only by the coordinates you provide.
Mapping result
Retained circular junction
Retained sequence
Open Circular DNA QC Open Validation Primer Designer