PHASE 15 · OPEN RESEARCH PLASMID DESIGNER

Design the construct, inspect every junction

Build or inspect a plasmid, visualize its circular map, screen common restriction sites, plan cloning primers and preserve a reproducible design record.

Research-use design aid. This page does not replace sequence verification, cloning-junction confirmation or experimental validation. Do not use it as a clinical or regulated diagnostic tool.

Construct definition

Paste a backbone, optionally add an insert, or import FASTA / GenBank.

Plasmid name Insertion position 0 = before first backbone base

Import FASTA / GenBank No file loaded. Circular plasmid Treat insert as coding sequence

Backbone sequence (5′→3′)0 bp Insert sequence (optional, 5′→3′)0 bp

Feature annotation

Add promoter, CDS, tag, terminator, origin, marker or other annotated regions. Coordinates refer to the backbone before insertion.

Feature	Type	Coordinates	Strand
No features added yet.

Cloning strategy

Generate research-oriented primer overhangs for a selected assembly strategy.

Strategy Forward enzyme Reverse enzyme Overlap length Type IIS enzyme Left 4-nt overhang Right 4-nt overhang Primer core length

Ready.

FINAL SIZE—

GC—

FEATURES—

UNIQUE CUTTERS—

STRATEGY—

Plasmid map

Circular feature map and construct fingerprint.

Restriction-site screen

Common research enzymes are scanned across the complete assembled sequence, including the circular origin junction.

Show unique cutters only

Enzyme	Recognition site	Count	Positions (1-based)	Status

Cloning-primer plan

Review overhangs, primer metrics and strategy-specific warnings before any experimental use.

A cloning strategy must be selected and the plasmid design rebuilt before a primer pair can be sent to Assay QC.

Design the construct, inspect every junction

Construct definition

Feature annotation

Cloning strategy

Plasmid map

Restriction-site screen

Cloning-primer plan

Construct QC

Sequence & export