Phase 68 ยท cloning utility
Ligation & Gibson / NEBuilder Calculator
Convert vector mass to pmol, calculate insert masses for chosen molar ratios, and optionally inspect a Gibson/NEBuilder overlap sequence and estimated Tm.
Vector
Insert fragments
Optional Gibson / NEBuilder overlap
Stoichiometry
| Fragment | Length | Ratio to vector | Target pmol | Recommended mass |
|---|---|---|---|---|
| No calculation yet. | ||||
Overlap assessment
Add an overlap sequence to calculate its Tm.