Standard PCR
Primer3 design, structural QC, local specificity and NCBI BLAST pre-screen.
RT-qPCR
Exon-aware ranking, gDNA-risk estimate, hydrolysis probe candidates and BLAST pre-screen.
Project Manager
Save multiple projects and keep a local design history.
Current project
1. Target selection
2. Application and sequence
3. Design parameters
1. Peptide / Protein sequence
2. Analysis results
3. Peptide Design
4. MHC / HLA Binding Prediction
Prioritize peptide candidates by predicted MHC/HLA binding. Binding prediction does not guarantee antigen presentation or immunogenicity.
Saved projects
Design history
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Current session
Free
10 designs / month
3 BLAST checks / month
5 PDF reports / month
Pro
500 designs / month
200 BLAST checks / month
500 PDF reports / month
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About this prototype
This is a research prototype for molecular assay design. It combines Primer3, NCBI transcript retrieval, exon-aware RT-qPCR ranking, hydrolysis-probe suggestions, local background screening, NCBI BLAST pre-screening, project history and PDF reports.