Bioinformatics · Phase 35 · RNAi workflow
siRNA Target Accessibility & Duplex Context Analyzer
After selecting the intended transcript in Phase 34, examine the local RNA structural context around each exact target site. The built-in calculation is explicitly a structural-accessibility proxy. True thermodynamic values are shown only when ViennaRNA is actually available.
Accessibility inputs
Use Phase 34 to choose the intended transcript isoform first.
Ready.
Target-site structural context
| Guide | Target site | Position | Target GC % | Local GC % | Pair loss | Normalized penalty | Accessibility proxy | Vienna MFE | MFE site unpaired % | Duplex ΔG | Model |
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