Bioinformatics · Phase 26
siRNA Designer & Local Off-target Pre-screen
Generate 21-nt siRNA candidates from an RNA or DNA target sequence, review transparent sequence-priority features, and optionally screen exact guide-seed and 17-nt complements against user-supplied background FASTA sequences.
Target input
DNA is converted T→U. The sequence is treated as the target transcript in 5′→3′ orientation.
Optional local pre-screen only. Exact complements are searched only in the sequences you provide here.
Ready for analysis.
Ranked siRNA candidates
| # | Target | Guide RNA 5′→3′ | Passenger 5′→3′ | GC% | Seed 2–8 | Asymmetry | BG seed | BG 17-mer | Priority | Flags |
|---|
Continue the siRNA workflow
After candidate generation, compare transcript-isoform coverage first, then review target-site structural context.