Bioinformatics · Phase 34 · RNAi workflow
siRNA Isoform Coverage Analyzer Continue from the existing siRNA Designer by mapping candidate guides across a supplied transcript-isoform set. This phase does not generate new siRNAs; it asks whether each existing candidate targets all, some, one, or none of the supplied isoforms.
Isoform coverage inputs siRNA guides
Transcript isoforms (FASTA)
Guide input represents Antisense siRNA guide Target-site / sense sequence
Near-match substitutions 0 — exact coverage only Up to 1 Up to 2
Max hits / guide / isoform
Load sample Analyze isoform coverage
Ready.
What this adds beyond Phase 26 maps existing siRNA candidates across multiple transcript isoforms reports exact target coordinates for each supplied isoform classifies pan-isoform, subset and isoform-selective exact targeting flags repeated exact target sites optionally reports substitution-only near matches Not a second siRNA designer. Candidate generation and first-pass sequence scoring remain in Phase 26.
Isoform coverage summary Guide Guide sequence Target site Exact isoforms Coverage % Exact hits Near-match isoforms Classification Warnings